Coral Sampling Encore – Desecheo Ridge, Puerto Rico | SOI Divestream 508

Welcome to ROV SuBastian Divestream #508! This is the six dive of the expedition, and we are excited to host a wonderful group of scientists lead by Dr. Colleen Hansel. Here is the Dive Plan!
1. Conduct measurements and collections as follows (in order):
a. Photogrammetry – image coral
b. SOLARIS – measure superoxide; start by measuring seawater ~1 m away from
the coral, move wand to surface, move wand to several other areas on the coral
surface, then move back to background seawater.
c. Squeezers – if collecting waters at surface, fire a squeezer at the coral surface
d. Collect coral fragments in coral quivers.

2. Conduct measurements and collections of a couple sponges as follows (in order):
a. SOLARIS – measure superoxide; start by measuring seawater ~1 m away from
the sponge, move wand to outside of sponge, move wand inside of sponge, then
move back to outside of sponge.
b. Squeezers – collect waters inside sponge
c. Collect sponge fragments and place in coral quiver

3. Collect eDNA samples and water samples (Niskins) at sites along transects
corresponding to ROS data.

4. Towards end of dive, collect coral fragments for aquaria incubations (one species,
multiple fragments)

5. End Dive. We hope you enjoy and ask us all of your burning questions!

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